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发现者/寻路者®；PD-1（SHP1）信号生物测定试剂盒/1000dp（10x96井）/93-1104Y19-00118
发现者/寻路者®；PD-1（SHP1）信号生物测定试剂盒/1000dp（10x96井）/93-1104Y19-00118
商品编号：
93-1104Y19-00118
市场价：
¥179380.00
美元价：
107628.00
产品分类：
细胞代谢
公司分类：
cellular_metabolism
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The PathHunter PD1 Signaling Bioassay consists of human cells engineered to stably express an ED-tagged immune checkpoint receptor, PD-1, while EA is fused to either the full length or the phosphotyrosine-binding SH2 domain of an intracellular signaling protein, SHP1. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal.The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.
- Target/Description
- Programmed cell death protein 1
- Group
- T cell receptor (inhibitory)
- SH2 Domain
- SHP1
- Cell Background
- Jurkat
- Species
- Human
- Accession Number
- NM_005018.2
- Agonist/Ligand
- Anti-PD1 Antibody [NAT105]
- Antagonist/Inhibitor
- Anti-PD1 antibody [NAT105]
- Assay Measures
- SH2 Recruitment
- Bioassay Target Name
- PD-1
- Assay Qualified With
- Keytruda®
- Drug Trademark Owner
- Keytruda®is a registered trademark of Merck & Co., Inc.
How it works
The graph is an example of a typical dose-response curve for the PD-1 Signaling Bioassay, generated usingthe protocol outlined in this user manual. The data shows a potent, dose-dependent inhibition of PD-1 activation in Jurkat cells with an anti-PD-1 antibody.
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